ABSTRACT
OBJECTIVE: The aim of our study was to analyze the in vitro activity of vancomycin and kanamycin combination against Staphylococcus aureus and Escherichia coli
STUDY DESIGN: It was an experimental study
PLACE AND DURATION: The study was conducted in the month of February, 2007 at the University of East London
METHODS: Checkerboard synergy tests were performed in broth microdilution trays
RESULTS: FIC [Fractional Inhibitory Concentration] of 0.625 was calculated for the two drugs against E. coli that showed additive effect making this combination not effective against this organism. FIC of 0.182 was observed when the same combination of drugs was tested against Staph. Aureus
CONCLUSION: Vancomycin and Kanamycin have synergistic effect on Staphylococcus aureus and Escherichia coli
ABSTRACT
Objective: to find out if the bacteria present in human oral cavity have the ability to produce azoreductase enzyme and to identify the types of bacteria from the human oral flora that can decolourise the azo dye amaranth
Design: experimental study
Materials and methods: human oral microbial flora were screened for their abilities to reduce azo compound by growing them on brain heart infusion agar plates containing 100 µµMolar of the azo dye amaranth. Bacteria that reduced amaranth were detected by the appearance of clear zones around the colonies. Bacteria were identified using standard microbiological techniques. Organisms belonging to Micrococcaceae family were further identified by API Staph kit from BioMérieux SA
Results: there was more decolourisation of the amaranth dye in the plates that were incubated anaerobically as compared to plates grown in aerobic conditions. Azoreduction of the amaranth dye proceeded at a slower rate when performed under aerobic condition as compared to when performed under anaerobic environment. Bacteria having azoreductase activity were identified as Neisseria flavescens, Lactobacillus, Staphylococcus hominis and Staphylococcus lentis
Conclusion: this study suggests that azo dyes present in food and drink can be reduced by some common oral microflora and the environment in the oral cavity is also favourable for the reduction of these compounds
ABSTRACT
We report a highly rare case of primitive neuroectodermal tumor of tongue. A 13-years old male presented with progressive lesion at the tip of tongue and the left lateral border since six months. Excision biopsy of the patient was done with local flap reconstruction of the tongue. Histopathology favoured the diagnosis of primitive neuroectodermal tumor